RBS
Part:BBa_K4137015:Design
Designed by: Jen-Hao Yao Group: iGEM22_GEMS_Taiwan (2022-10-08)
pET28a(+) RBS + 5' UTR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Initially, only the RBS was included in this RBS design. However, while recalling background knowledge of molecular biology, we remembered that a 5' UTR often precedes coding sections. We performed additional research and found that 5' UTRs serve the important purpose of improving overexpression. Thus, we included 6 arbitrary nucleotides after the RBS, making sure not to include an ATG sequence so as to prevent premature or frameshifted translation.